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3.8 The control of gene expression
Quick questions on Recombinant DNA technology - AQA A-Level Biology 3.8
4short Q&A pairs drawn directly from our worked dot-point answer. For full context and worked exam questions, read the parent dot-point page.
What are marker genes?Show answer
Only some cells take up the plasmid. A marker gene (for example antibiotic resistance, fluorescence or an enzyme that changes colour) is included so that transformed cells can be identified and selected.
What is q1?Show answer
Explain one advantage of using cDNA made by reverse transcriptase rather than cutting the gene from genomic DNA. [2 marks]
What is q2?Show answer
Calculate the number of DNA molecules produced from one starting molecule after 10 cycles of PCR. [1 mark]
What is q3?Show answer
Explain why a marker gene is needed when transforming bacteria with a plasmid. [2 marks]
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